Hexokinase 1 antibody (Middle Region)

Catalog No. ABIN2788662

The Rabbit Polyclonal anti-Hexokinase 1 antibody is suitable to detect Hexokinase 1 in samples from Human, Mouse, Rat, Dog, Cow, Pig, Rabbit, Horse and Guinea Pig. It has been validated for WB.

Quick Overview for Hexokinase 1 antibody (Middle Region) (ABIN2788662)

Target: Hexokinase 1 (HK1)

Reactivity: Human, Mouse, Rat, Dog, Cow, Pig, Rabbit, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Hexokinase 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Hexokinase 1 Antibody

Binding Specificity: Middle Region

Purpose: HK1 Antibody - middle region

Sequence: ILVKMAKEGL LFEGRITPEL LTRGKFNTSD VSAIEKNKEG LHNAKEILTR

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 79%, Horse: 93%, Human: 100%, Mouse: 77%, Pig: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against HK1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HK1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP61058-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Hexokinase 1

Target: Hexokinase 1 (HK1)

Alternative Name: HK1

Background:

Background Information: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus, the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein.

Gene Name: Hexokinase 1

Alternative Symbols: HK, HKD, HKI, HXK1, RP79, HMSNR, HK1-ta, HK1-tb, HK1-tc, NEDVIBA, hexokinase

Protein Name: Hexokinase-1

Molecular Weight: 103kDa

Gene ID: 3098

NCBI Accession: NP_277033

UniProt: P19367

Pathways: Carbohydrate Homeostasis, Warburg Effect