CD13 antibody (N-Term)

Catalog No. ABIN2788665

The Rabbit Polyclonal anti-CD13 antibody is suitable to detect CD13 in samples from Human, Rat, Horse, Guinea Pig, Dog and Rabbit. It has been validated for WB.

Quick Overview for CD13 antibody (N-Term) (ABIN2788665)

Target: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Reactivity: Human, Rat, Horse, Guinea Pig, Dog, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD13 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CD13 Antibody

Binding Specificity: N-Term

Purpose: ANPEP Antibody - N-terminal region

Sequence: VGGSQPPDID KTELVEPTEY LVVHLKGSLV KDSQYEMDSE FEGELADDLA

Predicted Reactivity: Dog: 79%, Guinea Pig: 85%, Horse: 86%, Human: 100%, Rabbit: 79%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against ANPEP. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ANPEP

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP61079-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for CD13

Target: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Alternative Name: ANPEP

Background:

Background Information: Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in this gene appear to be a cause of various types of leukemia or lymphoma.

Gene Name: Alanyl (membrane) aminopeptidase

Alternative Symbols: APN, CD13, LAP1, P150, PEPN, GP150

Protein Name: Aminopeptidase N

Molecular Weight: 106kDa

Gene ID: 290

NCBI Accession: NP_001141

UniProt: P15144

Pathways: Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones