HLA-DMA antibody (N-Term)

Catalog No. ABIN2788737

The Rabbit Polyclonal anti-HLA-DMA antibody is suitable to detect HLA-DMA in samples from Human, Rat, Rabbit and Dog. It has been validated for WB.

Quick Overview for HLA-DMA antibody (N-Term) (ABIN2788737)

Target: HLA-DMA (Major Histocompatibility Complex, Class II, DM alpha (HLA-DMA))

Reactivity: Human, Rat, Rabbit, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLA-DMA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HLA-DMA Antibody

Binding Specificity: N-Term

Sequence: GLSEAYDEDQ LFFFDFSQNT RVPRLPEFAD WAQEQGDAPA ILFDKEFCEW

Predicted Reactivity: Dog: 77%, Human: 100%, Rabbit: 82%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against HLA-DMA. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HLA-DMA

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 261 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for HLA-DMA

Target: HLA-DMA (Major Histocompatibility Complex, Class II, DM alpha (HLA-DMA))

Alternative Name: HLA-DMA

Background: HLA-DMA belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta chain (DMB), both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail.
Alias Symbols: D6S222E, DMA, HLADM, RING6
Protein Interaction Partner: B3GAT1, U2AF2, RUNX1, UBC, CD82, HLA-DRB1, HLA-DOA, HLA-DRA, HLA-DMB, CD74,
Protein Size: 261

Molecular Weight: 29 kDa

Gene ID: 3108

NCBI Accession: NM_006120, NP_006111

UniProt: Q31604

Pathways: Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response