Shugoshin antibody (C-Term)

Catalog No. ABIN2788840

This Rabbit Polyclonal antibody specifically detects Shugoshin in WB. It exhibits reactivity toward Human, Rat and Horse.

Quick Overview for Shugoshin antibody (C-Term) (ABIN2788840)

Target: Shugoshin (SGOL1) (Shugoshin 1 (SGOL1))

Reactivity: Human, Rat, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Shugoshin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Shugoshin Antibody

Binding Specificity: C-Term

Purpose: SGO1 Antibody - C-terminal region

Sequence: NSDRPVTRPL AKRALKYTDE KETEGSKPTK TPTTTPPETQ QSPHLSLKDI

Predicted Reactivity: Horse: 91%, Human: 100%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against SGOL1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human SGOL1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP61569-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Shugoshin

Target: Shugoshin (SGOL1) (Shugoshin 1 (SGOL1))

Alternative Name: SGO1

Background:

Background Information: The protein encoded by this gene is a member of the shugoshin family of proteins. This protein is thought to protect centromeric cohesin from cleavage during mitotic prophase by preventing phosphorylation of a cohesin subunit. Reduced expression of this gene leads to the premature loss of centromeric cohesion, mis-segregation of sister chromatids, and mitotic arrest. Evidence suggests that this protein also protects a small subset of cohesin found along the length of the chromosome arms during mitotic prophase. An isoform lacking exon 6 has been shown to play a role in the cohesion of centrioles (PMID: 16582621 and PMID:18331714). Mutations in this gene have been associated with Chronic Atrial and Intestinal Dysrhythmia (CAID) syndrome, characterized by the co-occurrence of Sick Sinus Syndrome (SSS) and Chronic Intestinal Pseudo-obstruction (CIPO) within the first four decades of life (PMID:25282101). Fibroblast cells from CAID patients exhibited both increased cell proliferation and higher rates of senescence. Pseudogenes of this gene have been found on chromosomes 1 and 7. Alternative splicing results in multiple transcript variants.

Gene Name: shugoshin 1

Alternative Symbols: SGO, CAID, SGOL1, NY-BR-85

Protein Name: shugoshin 1

Molecular Weight: 60kDa

Gene ID: 151648

NCBI Accession: NP_001012409

UniProt: B5BUA4

Pathways: Maintenance of Protein Location