EIF5B antibody (Middle Region)

Catalog No. ABIN2788862

The Rabbit Polyclonal anti-EIF5B antibody is suitable to detect EIF5B in samples from Human, Dog, Horse, Cow, Pig and Rabbit. It has been validated for WB.

Quick Overview for EIF5B antibody (Middle Region) (ABIN2788862)

Target: EIF5B (Eukaryotic Translation Initiation Factor 5B (EIF5B))

Reactivity: Human, Dog, Horse, Cow, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF5B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EIF5B Antibody

Binding Specificity: Middle Region

Purpose: EIF5B Antibody - middle region

Sequence: SEGSEGDEED EKVSDEKDSG KTLDKKPSKE MSSDSEYDSD DDRTKEERAY

Predicted Reactivity: Cow: 86%, Dog: 86%, Horse: 86%, Human: 100%, Pig: 93%, Rabbit: 93%

Characteristics: This is a rabbit polyclonal antibody against EIF5B. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP61644-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for EIF5B

Target: EIF5B (Eukaryotic Translation Initiation Factor 5B (EIF5B))

Alternative Name: EIF5B

Background:

Background Information: Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately.

Gene Name: Eukaryotic translation initiation factor 5B

Alternative Symbols: IF2

Protein Name: Eukaryotic translation initiation factor 5B

Molecular Weight: 139kDa

Gene ID: 9669

NCBI Accession: NP_056988

UniProt: O60841