EIF3F antibody (N-Term)

Catalog No. ABIN2788967

The Rabbit Polyclonal anti-EIF3F antibody is suitable to detect EIF3F in samples from Human, Mouse, Rat, Cow, Rabbit, Dog, Horse, Zebrafish (Danio rerio) and Guinea Pig. It has been validated for WB.

Quick Overview for EIF3F antibody (N-Term) (ABIN2788967)

Target: EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Horse, Zebrafish (Danio rerio), Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3F antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EIF3F Antibody

Binding Specificity: N-Term

Purpose: EIF3F Antibody - N-terminal region

Sequence: VILASIVDSY ERRNEGAARV IGTLLGTVDK HSVEVTNCFS VPHNESEDEV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against EIF3F. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP61970-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for EIF3F

Target: EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))

Alternative Name: EIF3F

Background:

Targeted Subunit: F

Background Information: EIF3F is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Gene Name: Eukaryotic translation initiation factor 3, subunit F

Alternative Symbols: MRT67, EIF3S5, eIF3-p47

Protein Name: Eukaryotic translation initiation factor 3 subunit F

Molecular Weight: 39kDa

Gene ID: 8665

NCBI Accession: NP_003745

UniProt: O00303

Pathways: Ribonucleoprotein Complex Subunit Organization