P4HA1 antibody (Middle Region)

Catalog No. ABIN2788990

The Rabbit Polyclonal anti-P4HA1 antibody (ABIN2788990) specifically detects P4HA1 in WB. The antibody is reactive with Human, Rat, Mouse, Dog, Pig, Cow, Horse, Rabbit and Guinea Pig samples.

Quick Overview for P4HA1 antibody (Middle Region) (ABIN2788990)

Target: P4HA1 (Prolyl 4-Hydroxylase, alpha Polypeptide I (P4HA1))

Reactivity: Human, Rat, Mouse, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P4HA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-P4HA1 Antibody

Binding Specificity: Middle Region

Purpose: P4HA1 Antibody - middle region

Sequence: MAKEKDVNKS ASDDQSDQKT TPKKKGVAVD YLPERQKYEM LCRGEGIKMT

Predicted Reactivity: Cow: 86%, Dog: 93%, Guinea Pig: 79%, Horse: 93%, Human: 100%, Mouse: 85%, Pig: 86%, Rabbit: 86%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against P4HA1. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP62017-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for P4HA1

Target: P4HA1 (Prolyl 4-Hydroxylase, alpha Polypeptide I (P4HA1))

Alternative Name: P4HA1

Background:

Targeted Subunit: alpha-1

Background Information: This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.

Gene Name: Prolyl 4-hydroxylase, alpha polypeptide I

Alternative Symbols: P4HA

Protein Name: Prolyl 4-hydroxylase subunit alpha-1 Ensembl ENSP00000414464

Molecular Weight: 56kDa

Gene ID: 5033

NCBI Accession: NP_001136068

UniProt: C9JL12