EEF1E1 antibody (N-Term)

Catalog No. ABIN2789165

Product Details anti-EEF1E1 Antibody

Target: EEF1E1 (Eukaryotic Translation Elongation Factor 1 epsilon 1 (EEF1E1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Horse, Rabbit, Guinea Pig, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEF1E1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: IPVLQTNNGP SLTGLTTIAA HLVKQANKEY LLGSTAEEKA IVQQWLEYRV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 86%, Rabbit: 100%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against EEF1E1. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 174 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for EEF1E1

Target: EEF1E1 (Eukaryotic Translation Elongation Factor 1 epsilon 1 (EEF1E1))

Alternative Name: EEF1E1 (EEF1E1 Products)

Background: This gene encodes a multifunctional protein that localizes to both the cytoplasm and nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage, and it plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream MUTED (muted homolog) gene. An EEF1E1-related pseudogene has been identified on chromosome 2.
Alias Symbols: AIMP3, P18
Protein Interaction Partner: NAT9, CALCOCO2, HUWE1, RPLP0, RPL27A, RPL26, RPL24, RPL23A, RPL21, RPL18, RPL15, RPL13, RPL7A, QARS, MARS, CCAR2, RPL36, AIMP1, IQGAP1, AIMP2, IARS, DNAJA1, HNRNPA2B1, EIF4G1, EIF2S3, EIF2S1, DARS, gag, UBC, ITGA4, RARS, KARS, EPRS, ATR, ATM,
Protein Size: 174

Molecular Weight: 19 kDa

Gene ID: 9521

NCBI Accession: NM_004280, NP_004271

UniProt: O43324

Pathways: Positive Regulation of Response to DNA Damage Stimulus