EEF1E1 antibody (N-Term)

Catalog No. ABIN2789165

This Rabbit Polyclonal antibody specifically detects EEF1E1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Horse, Rabbit, Guinea Pig and Dog.

Quick Overview for EEF1E1 antibody (N-Term) (ABIN2789165)

Target: EEF1E1 (Eukaryotic Translation Elongation Factor 1 epsilon 1 (EEF1E1))

Reactivity: Human, Mouse, Rat, Cow, Horse, Rabbit, Guinea Pig, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEF1E1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EEF1E1 Antibody

Binding Specificity: N-Term

Sequence: IPVLQTNNGP SLTGLTTIAA HLVKQANKEY LLGSTAEEKA IVQQWLEYRV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 86%, Rabbit: 100%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against EEF1E1. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 174 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for EEF1E1

Target: EEF1E1 (Eukaryotic Translation Elongation Factor 1 epsilon 1 (EEF1E1))

Alternative Name: EEF1E1

Background: This gene encodes a multifunctional protein that localizes to both the cytoplasm and nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage, and it plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream MUTED (muted homolog) gene. An EEF1E1-related pseudogene has been identified on chromosome 2.
Alias Symbols: AIMP3, P18
Protein Interaction Partner: NAT9, CALCOCO2, HUWE1, RPLP0, RPL27A, RPL26, RPL24, RPL23A, RPL21, RPL18, RPL15, RPL13, RPL7A, QARS, MARS, CCAR2, RPL36, AIMP1, IQGAP1, AIMP2, IARS, DNAJA1, HNRNPA2B1, EIF4G1, EIF2S3, EIF2S1, DARS, gag, UBC, ITGA4, RARS, KARS, EPRS, ATR, ATM,
Protein Size: 174

Molecular Weight: 19 kDa

Gene ID: 9521

NCBI Accession: NM_004280, NP_004271

UniProt: O43324

Pathways: Positive Regulation of Response to DNA Damage Stimulus