HLA-C antibody (C-Term)

Catalog No. ABIN2789353

The Rabbit Polyclonal anti-HLA-C antibody (ABIN2789353) specifically detects HLA-C in WB. The antibody is reactive with Human, Pig and Rabbit samples.

Quick Overview for HLA-C antibody (C-Term) (ABIN2789353)

Target: HLA-C (Major Histocompatibility Complex Class I C (HLA-C))

Reactivity: Human, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLA-C antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HLA-C Antibody

Binding Specificity: C-Term

Purpose: HLA-C Antibody - C-terminal region

Sequence: PPKTHVTHHP LSDHEATLRC WALGFYPAEI TLTWQRDGED QTQDTELVET

Predicted Reactivity: Human: 100%, Pig: 100%, Rabbit: 100%

Characteristics: This is a rabbit polyclonal antibody against HLA-C. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP63059-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for HLA-C

Target: HLA-C (Major Histocompatibility Complex Class I C (HLA-C))

Alternative Name: HLA-C

Background:

Background Information: HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Over one hundred HLA-C alleles have been described.

Gene Name: Major histocompatibility complex, class I, C

Alternative Symbols: MHC, HLAC, HLC-C, D6S204, PSORS1, HLA-JY3

Protein Name: HLA class I histocompatibility antigen, Cw-16 alpha chain

Molecular Weight: 38kDa

Gene ID: 3107

NCBI Accession: NP_002108

UniProt: Q29960

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response