Angiopoietin 2 antibody (Middle Region)

Catalog No. ABIN2789397

The Rabbit Polyclonal anti-Angiopoietin 2 antibody (ABIN2789397) specifically detects Angiopoietin 2 in WB. The antibody is reactive with Human, Mouse, Rat, Rabbit, Pig, Cow, Dog, Guinea Pig, Horse and Sheep samples.

Quick Overview for Angiopoietin 2 antibody (Middle Region) (ABIN2789397)

Target: Angiopoietin 2 (ANGPT2)

Reactivity: Human, Mouse, Rat, Rabbit, Pig, Cow, Dog, Guinea Pig, Horse, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Angiopoietin 2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Angiopoietin 2 Antibody

Binding Specificity: Middle Region

Purpose: Angpt2 Antibody - middle region

Sequence: NQTTRLELQL LQHSISTNKL EKQILDQTSE INKLQNKNSF LEQKVLDMEG

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 100%, Rat: 100%, Sheep: 93%

Characteristics: This is a rabbit polyclonal antibody against Angpt2. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Mouse Angpt2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP63170-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Angiopoietin 2

Target: Angiopoietin 2 (ANGPT2)

Alternative Name: Angpt2

Background:

Background Information: Angpt2 binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. It can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.

Gene Name: angiopoietin 2

Alternative Symbols: An, Ang, Ang2, Agpt2, Ang-2

Protein Name: Angiopoietin-2

Molecular Weight: 56kDa

Gene ID: 11601

NCBI Accession: NP_031452

UniProt: O35608

Pathways: RTK Signaling