UCP1 antibody (C-Term)

Catalog No. ABIN2789411

The Rabbit Polyclonal anti-UCP1 antibody (ABIN2789411) specifically detects UCP1 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Sheep, Guinea Pig and Horse samples.

Quick Overview for UCP1 antibody (C-Term) (ABIN2789411)

Target: UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))

Reactivity: Human, Mouse, Rat, Cow, Sheep, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCP1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-UCP1 Antibody

Binding Specificity: C-Term

Purpose: UCP1 Antibody - C-terminal region

Sequence: AMKVFTNEGP TAFFKGLVPS FLRLGSWNVI MFVCFEQLKR ELSKSRQTMD

Predicted Reactivity: Cow: 79%, Guinea Pig: 79%, Horse: 86%, Human: 100%, Mouse: 79%, Rat: 91%, Sheep: 86%

Characteristics: This is a rabbit polyclonal antibody against UCP1. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP63203-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for UCP1

Target: UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))

Alternative Name: UCP1

Background:

Background Information: Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat.

Gene Name: Uncoupling protein 1 (mitochondrial, proton carrier)

Alternative Symbols: UCP, SLC25A7

Protein Name: Mitochondrial brown fat uncoupling protein 1

Molecular Weight: 34kDa

Gene ID: 7350

NCBI Accession: NP_068605

UniProt: P25874

Pathways: Proton Transport, Brown Fat Cell Differentiation