DGAT1 antibody (N-Term)

Catalog No. ABIN2789488

This Rabbit Polyclonal antibody specifically detects DGAT1 in WB. It exhibits reactivity toward Human, Rat, Mouse, Dog, Cow, Pig, Sheep, Goat, Rabbit, Horse, Guinea Pig and Zebrafish (Danio rerio).

Quick Overview for DGAT1 antibody (N-Term) (ABIN2789488)

Target: DGAT1 (Diacylglycerol O-Acyltransferase 1 (DGAT1))

Reactivity: Human, Rat, Mouse, Dog, Cow, Pig, Sheep, Goat, Rabbit, Horse, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DGAT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DGAT1 Antibody

Binding Specificity: N-Term

Purpose: Dgat1 Antibody - N-terminal region

Sequence: HRLQDSLFSS DSGFSNYRGI LNWCVVMLIL SNARLFLENL IKYGILVDPI

Predicted Reactivity: Cow: 100%, Dog: 93%, Goat: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against Dgat1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Mouse Dgat1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP63409-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for DGAT1

Target: DGAT1 (Diacylglycerol O-Acyltransferase 1 (DGAT1))

Alternative Name: Dgat1

Background:

Background Information: Dgat1 catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. It may be involved in VLDL (very low density lipoprotein) assembly. In liver, Dgat1 plays a role in esterifying exogenous fatty acids to glycerol. It functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders.

Gene Name: diacylglycerol O-acyltransferase 1

Alternative Symbols: ARAT, Dgat, C75990, D15Ertd23, D15Ertd23e

Protein Name: Diacylglycerol O-acyltransferase 1

Molecular Weight: 57kDa

Gene ID: 13350

NCBI Accession: NP_034176

UniProt: Q9Z2A7

Pathways: Hormone Transport