SPHK1 antibody (Middle Region)

Catalog No. ABIN2789510

This anti-SPHK1 antibody is a Rabbit Polyclonal antibody detecting SPHK1 in WB. Suitable for Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Goat and Rabbit.

Quick Overview for SPHK1 antibody (Middle Region) (ABIN2789510)

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Goat, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPHK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SPHK1 Antibody

Binding Specificity: Middle Region

Sequence: DVDLESEKYR RLGEMRFTLG TFLRLAALRT YRGRLAYLPV GRVGSKTPAS

Predicted Reactivity: Cow: 93%, Dog: 93%, Goat: 77%, Guinea Pig: 86%, Horse: 86%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against SPHK1. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 384 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SPHK1

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Alternative Name: SPHK1

Background: The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: SPHK
Protein Interaction Partner: UBC, BIRC3, MBTPS1, TRAF6, RARG, EP300, SPHKAP, RPS6KC1, CTNND2, TRAF2, LYN, FYN, CTSB,
Protein Size: 384

Molecular Weight: 42 kDa

Gene ID: 8877

NCBI Accession: NM_001142601, NP_001136073

UniProt: Q9NYA1

Pathways: VEGF Signaling