PDE6B antibody (N-Term)

Catalog No. ABIN2789554

The Rabbit Polyclonal anti-PDE6B antibody is suitable to detect PDE6B in samples from Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Rabbit and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for PDE6B antibody (N-Term) (ABIN2789554)

Target: PDE6B (phosphodiesterase 6B, CGMP-Specific, Rod, beta (PDE6B))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDE6B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PDE6B Antibody

Binding Specificity: N-Term

Purpose: PDE6B Antibody - N-terminal region

Sequence: LSMPIVNKKE EIVGVATFYN RKDGKPFDEQ DEVLMESLTQ FLGWSVMNTD

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against PDE6B. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP63581-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PDE6B

Target: PDE6B (phosphodiesterase 6B, CGMP-Specific, Rod, beta (PDE6B))

Alternative Name: PDE6B

Background:

Targeted Subunit: beta

Background Information: Photon absorption triggers a signaling cascade in rod photoreceptors that activates cGMP phosphodiesterase (PDE), resulting in the rapid hydrolysis of cGMP, closure of cGMP-gated cation channels, and hyperpolarization of the cell. PDE is a peripheral membrane heterotrimeric enzyme made up of alpha, beta, and gamma subunits. This gene encodes the beta subunit. Mutations in this gene result in retinitis pigmentosa and autosomal dominant congenital stationary night blindness. Multiple transcript variants encoding different isoforms have been found for this gene.

Gene Name: Phosphodiesterase 6B, cGMP-specific, rod, beta

Alternative Symbols: rd1, PDEB, RP40, CSNB3, CSNBAD2, GMP-PDEbeta

Protein Name: Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta

Molecular Weight: 63kDa

Gene ID: 5158

NCBI Accession: NP_001138764

UniProt: E7ETT3

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction