PRKAG3 antibody (N-Term)

Catalog No. ABIN2789710

This Rabbit Polyclonal antibody specifically detects PRKAG3 in WB. It exhibits reactivity toward Human.

Quick Overview for PRKAG3 antibody (N-Term) (ABIN2789710)

Target: PRKAG3 (Protein Kinase, AMP-Activated, gamma 3 Non-Catalytic Subunit (PRKAG3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAG3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PRKAG3 Antibody

Binding Specificity: N-Term

Purpose: PRKAG3 Antibody - N-terminal region

Sequence: ENSSSWPSPA VTSSSERIRG KRRAKALRWT RQKSVEEGEP PGQGEGPRSR

Predicted Reactivity: Human: 100%

Characteristics: This is a rabbit polyclonal antibody against PRKAG3. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP64048-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PRKAG3

Target: PRKAG3 (Protein Kinase, AMP-Activated, gamma 3 Non-Catalytic Subunit (PRKAG3))

Alternative Name: PRKAG3

Background:

Targeted Subunit: gamma-3

Background Information: The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. It is dominantly expressed in skeletal muscle. Studies of the pig counterpart suggest that this subunit may play a key role in the regulation of energy metabolism in skeletal muscle.

Gene Name: Protein kinase, AMP-activated, gamma 3 non-catalytic subunit

Alternative Symbols: AMPKG3, SMGMQTL

Protein Name: 5'-AMP-activated protein kinase subunit gamma-3

Molecular Weight: 54kDa

Gene ID: 53632

NCBI Accession: NP_059127

UniProt: Q9UGI9

Pathways: AMPK Signaling, Cellular Glucan Metabolic Process, Warburg Effect