Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen
This PRKAG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 148-178 amino acids from the Central region of human PRKAG3.
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date
6 months
Okoshi, Ohta, Ishikawa, Sato, Kizaki: "Expression of 5'-AMP-activated protein kinase with starvation in murine thymocytes." in: The Bulletin of Tokyo Dental College, Vol. 52, Issue 1, pp. 21-9, (2011) (PubMed).
Fukuyama, Ohta, Okoshi, Suehara, Kizaki, Nakagawa: "Hypoxia induces expression and activation of AMPK in rat dental pulp cells." in: Journal of dental research, Vol. 86, Issue 9, pp. 903-7, (2007) (PubMed).
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Background
PRKAG3 is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. It is dominantly expressed in skeletal muscle. Studies of the pig counterpart suggest that this subunit may play a key role in the regulation of energy metabolism in skeletal muscle.