BRCC3 antibody (C-Term)

Catalog No. ABIN2790082

The Rabbit Polyclonal anti-BRCC3 antibody (ABIN2790082) specifically detects BRCC3 in WB. The antibody is reactive with Human, Mouse, Rat, Horse, Pig, Cow, Guinea Pig, Rabbit and Dog samples.

Quick Overview for BRCC3 antibody (C-Term) (ABIN2790082)

Target: BRCC3 (BRCA1/BRCA2-Containing Complex, Subunit 3 (BRCC3))

Reactivity: Human, Mouse, Rat, Horse, Pig, Cow, Guinea Pig, Rabbit, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRCC3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BRCC3 Antibody

Binding Specificity: C-Term

Purpose: BRCC3 Antibody - C-terminal region

Sequence: QERYIERAQQ EKEELQQEMA QQSRSLQQAQ QQLEEVRQNR QRADEDVEAA

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 83%, Horse: 93%, Human: 100%, Mouse: 93%, Pig: 90%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against DEF6. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of human BRCC3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP65234-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for BRCC3

Target: BRCC3 (BRCA1/BRCA2-Containing Complex, Subunit 3 (BRCC3))

Alternative Name: BRCC3

Background:

Background Information: This gene encodes a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. This complex plays a role in the DNA damage response, where it is responsible for the stable accumulation of BRCA1 at DNA break sites. The component encoded by this gene can specifically cleave Lys 63-linked polyubiquitin chains, and it regulates the abundance of these polyubiquitin chains in chromatin. The loss of this gene results in abnormal angiogenesis and is associated with syndromic moyamoya, a cerebrovascular angiopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 5.

Gene Name: BRCA1/BRCA2-containing complex subunit 3

Alternative Symbols: C6.1A, BRCC36, CXorf53

Protein Name: lys-63-specific deubiquitinase BRCC36

Molecular Weight: 41kDa

Gene ID: 79184

NCBI Accession: NP_001018065

UniProt: P46736

Pathways: Positive Regulation of Response to DNA Damage Stimulus