MOB1A antibody (C-Term)

Catalog No. ABIN2790218

This Rabbit Polyclonal antibody specifically detects MOB1A in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Dog and Zebrafish (Danio rerio).

Quick Overview for MOB1A antibody (C-Term) (ABIN2790218)

Target: MOB1A (MOBKL1B) (MOB1, Mps One Binder Kinase Activator-Like 1B (MOBKL1B))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Dog, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MOB1A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MOB1A Antibody

Binding Specificity: C-Term

Purpose: MOB1A Antibody - C-terminal region

Sequence: FDSVMQLQEE AHLNTSFKHF IFFVQEFNLI DRRELAPLQE LIEKLGSKDR

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against MOB1A. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human MOB1A

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP65686-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MOB1A

Target: MOB1A (MOBKL1B) (MOB1, Mps One Binder Kinase Activator-Like 1B (MOBKL1B))

Alternative Name: MOB1A

Background:

Background Information: MOB1A is an activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. MOB1A stimulates the kinase activity of STK38 and STK38L. MOB1A acts cooperatively with STK3/MST2 to activate STK38.

Gene Name: MOB kinase activator 1A

Alternative Symbols: MOB1, MATS1, Mob4B, C2orf6, MOBK1B, MOBKL1B

Protein Name: MOB kinase activator 1A

Molecular Weight: 25kDa

Gene ID: 55233

NCBI Accession: NP_060691

UniProt: Q9H8S9