PIK3C2A antibody (C-Term)

Catalog No. ABIN2790304

The Rabbit Polyclonal anti-PIK3C2A antibody is suitable to detect PIK3C2A in samples from Human, Mouse, Rat, Pig, Dog, Horse, Rabbit and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for PIK3C2A antibody (C-Term) (ABIN2790304)

Target: PIK3C2A (Phosphoinositide-3-Kinase, Class 2, alpha Polypeptide (PIK3C2A))

Reactivity: Human, Mouse, Rat, Pig, Dog, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIK3C2A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PIK3C2A Antibody

Binding Specificity: C-Term

Purpose: PIK3C2A Antibody - C-terminal region

Sequence: PNPYVKTYLL PDNHKTSKRK TKISRKTRNP TFNEMLVYSG YSKETLRQRE

Predicted Reactivity: Dog: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Pig: 93%, Rabbit: 93%, Rat: 93%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against PIK3C2A. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of human PIK3C2A

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP65938-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PIK3C2A

Target: PIK3C2A (Phosphoinositide-3-Kinase, Class 2, alpha Polypeptide (PIK3C2A))

Alternative Name: PIK3C2A

Background:

Background Information: The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling.

Gene Name: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha

Alternative Symbols: CPK, OCSKD, PI3-K-C2A, PI3K-C2alpha, PI3K-C2-alpha, PI3-K-C2(ALPHA)

Protein Name: phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha

Molecular Weight: 143kDa

Gene ID: 5286

NCBI Accession: NP_002636

UniProt: O00443

Pathways: EGFR Signaling Pathway, Inositol Metabolic Process, Platelet-derived growth Factor Receptor Signaling