Keratin 77 antibody (C-Term)

Catalog No. ABIN2790417

The Rabbit Polyclonal anti-Keratin 77 antibody is suitable to detect Keratin 77 in samples from Human, Rat, Mouse, Dog, Rabbit, Cow, Horse, Pig and Sheep. It has been validated for WB.

Quick Overview for Keratin 77 antibody (C-Term) (ABIN2790417)

Target: Keratin 77 (KRT77)

Reactivity: Human, Rat, Mouse, Dog, Rabbit, Cow, Horse, Pig, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Keratin 77 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Keratin 77 Antibody

Binding Specificity: C-Term

Purpose: KRT77 Antibody - C-terminal region

Sequence: VRTQYELIAQ RSKDEAEALY QTKYQELQIT AGRHGDDLKN SKMEIAELNR

Predicted Reactivity: Cow: 92%, Dog: 92%, Horse: 92%, Human: 100%, Mouse: 93%, Pig: 92%, Rabbit: 92%, Rat: 93%, Sheep: 92%

Characteristics: This is a rabbit polyclonal antibody against KRT77. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human KRT77

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP66387-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Keratin 77

Target: Keratin 77 (KRT77)

Alternative Name: KRT77

Background:

Background Information: Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. This gene encodes an epithelial keratin that is expressed in the skin and eccrine sweat glands. The type II keratins are clustered in a region of chromosome 12q13.

Alternative Symbols: K1B, KRT1B

Protein Name: Keratin, type II cytoskeletal 1b

Molecular Weight: 62kDa

Gene ID: 374454

NCBI Accession: NP_778253

UniProt: Q7Z794