MRPL32 antibody (C-Term)

Catalog No. ABIN2790818

This Rabbit Polyclonal antibody specifically detects MRPL32 in WB. It exhibits reactivity toward Human, Mouse, Sheep, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit and Rat.

Quick Overview for MRPL32 antibody (C-Term) (ABIN2790818)

Target: MRPL32 (Mitochondrial Ribosomal Protein L32 (MRPL32))

Reactivity: Human, Mouse, Sheep, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPL32 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MRPL32 Antibody

Binding Specificity: C-Term

Purpose: MRPL32 Antibody - C-terminal region

Sequence: CYEKVCKETA EIRRQIGKQE GGPFKAPTIE TVVLYTGETP SEQDQGKRII

Predicted Reactivity: Cow: 92%, Dog: 86%, Guinea Pig: 93%, Horse: 79%, Human: 100%, Mouse: 86%, Pig: 100%, Rabbit: 93%, Rat: 86%, Sheep: 92%

Characteristics: This is a rabbit polyclonal antibody against MRPL32. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human MRPL32

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP67674-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MRPL32

Target: MRPL32 (Mitochondrial Ribosomal Protein L32 (MRPL32))

Alternative Name: MRPL32

Background:

Background Information: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp.

Alternative Symbols: L32mt, HSPC283, MRP-L32, bMRP-59b

Protein Name: 39S ribosomal protein L32, mitochondrial

Molecular Weight: 21kDa

Gene ID: 64983

NCBI Accession: NP_114109

UniProt: Q9BYC8