MRPL32 antibody (AA 70-150)

Catalog No. ABIN7224340

This Rabbit Polyclonal antibody specifically detects MRPL32 in WB, ELISA, IF and IHC. It exhibits reactivity toward Human and Mouse.

Quick Overview for MRPL32 antibody (AA 70-150) (ABIN7224340)

Target: MRPL32 (Mitochondrial Ribosomal Protein L32 (MRPL32))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPL32 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-MRPL32 Antibody

Binding Specificity: AA 70-150

Purpose: Rabbit Anti-MRP-L32 Polyclonal Antibody

Specificity: MRP-L32 Polyclonal Antibody detects endogenous levels of MRP-L32 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human MRP-L32 at AA range: 70-150

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MRPL32

Target: MRPL32 (Mitochondrial Ribosomal Protein L32 (MRPL32))

Alternative Name: MRP-L32

Background: MRPL32, HSPC283, 39S ribosomal protein L32, mitochondrial, L32mt, MRP-L32Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPL32 (mitochondrial ribosomal protein L32) encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to MRPL32 is found on chromosome Xp.

Molecular Weight: 21kD

Gene ID: 64983

UniProt: Q9BYC8