MRPS25 antibody (C-Term)

Catalog No. ABIN2791675

The Rabbit Polyclonal anti-MRPS25 antibody (ABIN2791675) specifically detects MRPS25 in WB. The antibody is reactive with Human, Mouse, Rat, Horse, Rabbit, Cow, Dog and Guinea Pig samples.

Quick Overview for MRPS25 antibody (C-Term) (ABIN2791675)

Target: MRPS25 (Mitochondrial Ribosomal Protein S25 (MRPS25))

Reactivity: Human, Mouse, Rat, Horse, Rabbit, Cow, Dog, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPS25 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MRPS25 Antibody

Binding Specificity: C-Term

Purpose: MRPS25 Antibody - C-terminal region

Sequence: YKNPWVQIMM FKNMTPSPFL RFYLDSGEQV LVDVETKSNK EIMEHIRKIL

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 79%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against MRPS25. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of human MRPS25

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP70895-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MRPS25

Target: MRPS25 (Mitochondrial Ribosomal Protein S25 (MRPS25))

Alternative Name: MRPS25

Background:

Background Information: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4.

Gene Name: mitochondrial ribosomal protein S25

Alternative Symbols: RPMS25, COXPD50, MRP-S25

Protein Name: 28S ribosomal protein S25, mitochondrial

Molecular Weight: 12kDa

Gene ID: 64432

NCBI Accession: NP_071942

UniProt: P82663

Pathways: SARS-CoV-2 Protein Interactome