NLRC4 antibody (C-Term)

Catalog No. ABIN2792097

The Rabbit Polyclonal anti-NLRC4 antibody has been validated for WB and IHC. It is suitable to detect NLRC4 in samples from Human, Mouse, Rat and Guinea Pig. There is 1 publication available.

Quick Overview for NLRC4 antibody (C-Term) (ABIN2792097)

Target: NLRC4 (NLR Family, CARD Domain Containing 4 (NLRC4))

Reactivity: Human, Mouse, Rat, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NLRC4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NLRC4 Antibody

Binding Specificity: C-Term

Sequence: QLNLAGNRVS SDGWLAFMGV FENLKQLVFF DFSTKEFLPD PALVRKLSQV

Specificity: This antibody will recognize NLRC4 isoform 1 (116kD) and isoform 2 (40kD).

Predicted Reactivity: Guinea Pig: 80%, Human: 100%, Mouse: 86%, Rat: 80%

Characteristics: This is a rabbit polyclonal antibody against NLRC4. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human NLRC4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1024 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-NLRC4 antibody (ABIN2792097)

Hughes, Vivar, Kennis, Pratt-Thomas, Lowe, Shaner, Nietert, Spruill, Purves: "Inflammasomes are important mediators of cyclophosphamide-induced bladder inflammation." in: American journal of physiology. Renal physiology, Vol. 306, Issue 3, pp. F299-308, (2014) (PubMed).

Target Details for NLRC4

Target: NLRC4 (NLR Family, CARD Domain Containing 4 (NLRC4))

Alternative Name: NLRC4

Background: In C. elegans, Ced4 binds and activates Ced3, an apoptotic initiator caspase, via caspase-associated recruitment domains (CARDs). Human Ced4 homologs include APAF1, NOD1, and NOD2. These proteins have at least 1 N-terminal CARD domain followed by a centrally located nucleotide-binding domain (NBD or NACHT) and a C-terminal regulatory domain, found only in mammals, that contains either WD40 repeats or leucine-rich repeats (LRRs). CARD12 is a member of the Ced4 family and can induce apoptosis.
Alias Symbols: CLAN, IPAF, CLAN1, CLANA, CLANB, CLANC, CLAND, CARD12, CLR2.1
Protein Interaction Partner: UBC, XK, ALB, NLRC4, PSMC5, CASP8, NLRP4, PYCARD, NOD2, BCL10, CASP1, NLRP1, NLRP3, NOD1, NAIP,
Protein Size: 1024

Molecular Weight: 116 kDa

Gene ID: 58484

NCBI Accession: NM_021209, NP_067032

UniProt: Q9NPP4

Pathways: Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome