FADD antibody (C-Term)

Catalog No. ABIN2792110

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Quick Overview for FADD antibody (C-Term) (ABIN2792110)

Target: FADD (Fas (TNFRSF6)-Associated Via Death Domain (FADD))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FADD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-FADD Antibody

Binding Specificity: C-Term

Sequence: AHLVGALRSC QMNLVADLVQ EVQQARDLQN RSGAMSPMSW NSDASTSEAS

Predicted Reactivity: Human: 100%

Characteristics: This is a rabbit polyclonal antibody against FADD. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human FADD

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 208 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-FADD antibody (ABIN2792110)

Thomas, Henson, Reed, Salsbury, Thorburn: "Direct binding of Fas-associated death domain (FADD) to the tumor necrosis factor-related apoptosis-inducing ligand receptor DR5 is regulated by the death effector domain of FADD." in: The Journal of biological chemistry, Vol. 279, Issue 31, pp. 32780-5, (2004) (PubMed).

Target Details for FADD

Target: FADD (Fas (TNFRSF6)-Associated Via Death Domain (FADD))

Alternative Name: FADD

Background: The protein encoded by FADD is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade.
Alias Symbols: GIG3, MORT1
Protein Interaction Partner: CFLAR, CASP10, CASP8, ATG5, ATG12, TNF, TRADD, UBC, RIPK1, FAS, TNFSF10, SPOP, TNFRSF1A, MYD88, TNFRSF10A, TNFRSF10B, MKRN1, RIPK3, CUL3, TRAF2, PARK7, FASLG, LRRK2, IKBKG, BMX, FAF1, EDAR, DAPK1, CLIP3, DAXX, TNFAIP3, MAVS, KHDRBS1, TAB1, EZR, TRIM21, IR
Protein Size: 208

Molecular Weight: 23 kDa

Gene ID: 8772

NCBI Accession: NM_003824, NP_003815

UniProt: Q13158

Pathways: Apoptosis, TLR Signaling, Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades