CDK9 antibody (N-Term)

Catalog No. ABIN2792148

This anti-CDK9 antibody is a Rabbit Polyclonal antibody detecting CDK9 in WB. Suitable for Human, Mouse, Rat, Cow, Pig, Dog and Zebrafish (Danio rerio).

Quick Overview for CDK9 antibody (N-Term) (ABIN2792148)

Target: CDK9 (Cyclin-Dependent Kinase 9 (CDK9))

Reactivity: Human, Mouse, Rat, Cow, Pig, Dog, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK9 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CDK9 Antibody

Binding Specificity: N-Term

Sequence: PFCDEVSKYE KLAKIGQGTF GEVFKARHRK TGQKVALKKV LMENEKEGFP

Predicted Reactivity: Cow: 92%, Dog: 100%, Human: 100%, Mouse: 92%, Pig: 92%, Rat: 92%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against CDK9. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CDK9

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 372 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for CDK9

Target: CDK9 (Cyclin-Dependent Kinase 9 (CDK9))

Alternative Name: CDK9

Background: CDK9 is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.
Alias Symbols: RP11-228B15.5, C-2k, CDC2L4, PITALRE, TAK, CTK1
Protein Interaction Partner: UBC, MEPCE, tat, LARP7, BRD4, CCNT1, ATM, SUMO2, AFF1, HEXIM1, FKBP5, RNF20, MSL2, RNF40, HIST2H2BE, POLR2A, MSL1, CDK9, SMC2, PLEC, CCAR2, SMC4, STIP1, MLLT3, MLLT1, CDK12, AFF4, FGFR1, CCNT2, AFF3, FBXO25, CUL1, SKIV2L, ATP1A1, SUPT5H, RPS4X, RN7SK, HEX
Protein Size: 372

Molecular Weight: 43 kDa

Gene ID: 1025

NCBI Accession: NM_001261, NP_001252

UniProt: P50750

Pathways: Cell Division Cycle