CDC25C antibody (N-Term)

Catalog No. ABIN2792164

This Rabbit Polyclonal antibody specifically detects CDC25C in WB. It exhibits reactivity toward Human, Mouse and Rabbit.

Quick Overview for CDC25C antibody (N-Term) (ABIN2792164)

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Reactivity: Human, Mouse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25C antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CDC25C Antibody

Binding Specificity: N-Term

Sequence: QKYEKLEKIG EGTYGTVFKA KNRETHEIVA LKRVRLDDDD EGVPSSALRE

Predicted Reactivity: Human: 100%, Mouse: 91%, Rabbit: 83%

Characteristics: This is a rabbit polyclonal antibody against CDC25C. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CDC25C

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 400 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for CDC25C

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Alternative Name: CDC25C

Background: CDC25C gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.
Alias Symbols: CDC25, PPP1R60
Protein Interaction Partner: CDC37, UBC, HSP90AA1, HSPA4, YWHAB, YWHAQ, YWHAZ, YWHAH, YWHAG, YWHAE, PKN1, SFN, MAPK9, PLK1, CHEK1, CHEK2, CDS1, MARK3, BRSK2, CDK2, PCNA, Cdk1, USP17L2, MAPK3, MAPK1, BRSK1, LZTS1, PLK3, NEDD4, PIN1, MAPK14, TP53, HRAS, LCK, CCNB1, CCNA2, SRC, SP1,
Protein Size: 400

Molecular Weight: 45 kDa

Gene ID: 995

NCBI Accession: NM_022809, NP_073720

UniProt: P30307

Pathways: Cell Division Cycle, M Phase