Cyclin D3 antibody (C-Term)

Catalog No. ABIN2792167

The Rabbit Polyclonal anti-Cyclin D3 antibody has been validated for WB and IHC. It is suitable to detect Cyclin D3 in samples from Human, Mouse, Rat, Cow, Dog, Pig, Sheep and Guinea Pig.

Quick Overview for Cyclin D3 antibody (C-Term) (ABIN2792167)

Target: Cyclin D3 (CCND3)

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Sheep, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin D3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Cyclin D3 Antibody

Binding Specificity: C-Term

Sequence: CQEQIEAALR ESLREASQTS SSPAPKAPRG SSSQGPSQTS TPTDVTAIHL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 92%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against CCND3. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human CCND3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 292 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Cyclin D3

Target: Cyclin D3 (CCND3)

Alternative Name: CCND3

Background: CCND3 encodes a protein that belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation.
Protein Interaction Partner: POLR3GL, TFIP11, CDKN1A, CDK6, CDK4, CDK5, MCM10, CDKN1B, CDK2, CDK11B, FAS, SIL1, EIF3K, ATF5, VDR, MAPK6, NME1, MYB, GC, ETV1, RBL2, POLD1, CDK14, APP, RB1, UBC, PPP1CC, PPP1CB, PPP1CA, NCOA2, MAF1, NPDC1, DMTF1, CRABP2, RARA, DTNBP1, AKAP8, EFEMP2, CAS
Protein Size: 292

Molecular Weight: 33 kDa

Gene ID: 896

NCBI Accession: NM_001760, NP_001751

UniProt: P30281

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Glycosaminoglycan Metabolic Process