RBX1 antibody (Middle Region)

Catalog No. ABIN2792171

This Rabbit Polyclonal antibody specifically detects RBX1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Zebrafish (Danio rerio) and Guinea Pigand has been mentioned in 1 publication.

Quick Overview for RBX1 antibody (Middle Region) (ABIN2792171)

Target: RBX1 (Ring-Box 1, E3 Ubiquitin Protein Ligase (RBX1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Zebrafish (Danio rerio), Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RBX1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RBX1 Antibody

Binding Specificity: Middle Region

Sequence: NQASATSEEC TVAWGVCNHA FHFHCISRWL KTRQVCPLDN REWEFQKYGH

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against RBX1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RBX1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 108 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-RBX1 antibody (ABIN2792171)

Maerki, Olma, Staubli, Steigemann, Gerlich, Quadroni, Sumara, Peter: "The Cul3-KLHL21 E3 ubiquitin ligase targets aurora B to midzone microtubules in anaphase and is required for cytokinesis." in: The Journal of cell biology, Vol. 187, Issue 6, pp. 791-800, (2009) (PubMed).

Target Details for RBX1

Target: RBX1 (Ring-Box 1, E3 Ubiquitin Protein Ligase (RBX1))

Alternative Name: RBX1

Background: ROC1 encodes an evolutionarily conserved protein that interacts with cullins. The protein plays a unique role in the ubiquitination reaction by heterodimerizing with cullin-1 to catalyze ubiquitin polymerization. It also may be involved in the regulation of protein turn-over.
Alias Symbols: ROC1, RNF75, BA554C12.1
Protein Interaction Partner: CDT1, CDC34, KEAP1, UBE2L6, UBE2M, CUL1, CUL3, CUL4B, UBE2D3, UBE2D2, UBE2D1, DAB2IP, UBC, TP53, PRAME, DCUN1D1, CUL2, DCUN1D3, MAP4K1, VHL, NEDD4, MTSS1, VRK2, HIST1H4A, GFPT1, CTNNB1, CCNE1, DCUN1D5, FBXL5, RHOBTB3, SAMHD1, UBC12, FBXL15, UBE2R2, SERTAD
Protein Size: 108

Molecular Weight: 12 kDa

Gene ID: 9978

NCBI Accession: NM_014248, NP_055063

UniProt: P62877

Pathways: Cell Division Cycle, M Phase, SARS-CoV-2 Protein Interactome