E2F1 antibody (C-Term)

Catalog No. ABIN2792180

The Rabbit Polyclonal anti-E2F1 antibody has been validated for WB. It is suitable to detect E2F1 in samples from Human, Mouse, Rat, Dog, Guinea Pig, Rabbit and Horse.

Quick Overview for E2F1 antibody (C-Term) (ABIN2792180)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Rabbit, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-E2F1 Antibody

Binding Specificity: C-Term

Sequence: REDFSGLLPE EFISLSPPHE ALDYHFGLEE GEGIRDLFDC DFGDLTPLDF

Predicted Reactivity: Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against E2F1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human E2F1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 437 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F1

Background: The protein encoded by E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
Alias Symbols: RBP3, E2F-1, RBAP1, RBBP3
Protein Interaction Partner: RB1, SIRT1, KDM2A, LAMTOR5, EP300, KAT2B, VHL, RARA, STAT1, NCOR2, STOML1, RBL1, MAGEA11, SKP2, UHRF2, UBC, GTF2H1, ERCC3, SPT15, TFB1, TBP, NCOA3, CEBPE, PARP1, PRMT2, RBL2, CCNE1, TFDP2, TFDP1, CDKN2A, CEBPD, CDK2, CDK1, NEDD8, MCPH1, DDB2, DDB1, XIAP,
Protein Size: 437

Molecular Weight: 47 kDa

Gene ID: 1869

NCBI Accession: NM_005225, NP_005216

UniProt: Q01094

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy