Nanog antibody (N-Term)

Catalog No. ABIN2792522

Cited in 1 publication. The Rabbit Polyclonal anti-Nanog antibody (ABIN2792522) specifically detects Nanog in WB. The antibody is reactive with Human and Pig samples.

Quick Overview for Nanog antibody (N-Term) (ABIN2792522)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivity: Human, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nanog antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Nanog Antibody

Binding Specificity: N-Term

Sequence: PMPVICGPEE NYPSLQMSSA EMPHTETVSP LPSSMDLLIQ DSPDSSTSPK

Cross-Reactivity: Human, Pig (Porcine)

Predicted Reactivity: Human: 100%, Pig: 92%

Characteristics: This is a rabbit polyclonal antibody against NANOG. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NANOG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Antigen size: 305 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Nanog antibody (ABIN2792522)

Yang, Atkinson, Vilella, Lloret, Armstrong, Mann, Lako: "Opposing putative roles for canonical and noncanonical NF?B signaling on the survival, proliferation, and differentiation potential of human embryonic stem cells." in: Stem cells (Dayton, Ohio), Vol. 28, Issue 11, pp. 1970-80, (2010) (PubMed).

Target Details for Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Alternative Name: NANOG

Background: NANOG is a new marker for testicular carcinoma in situ and germ cell tumors. Gene knockdown of Nanog promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal.
Alias Symbols: -
Protein Interaction Partner: DOT1L, UBC, ZNF281, RHOXF2, SALL4, MED12,
Protein Size: 305

Molecular Weight: 35 kDa

Gene ID: 79923

NCBI Accession: NM_024865, NP_079141

UniProt: Q6NSW7

Pathways: Stem Cell Maintenance