LEF1 antibody (N-Term)

Catalog No. ABIN2792635

The Rabbit Polyclonal anti-LEF1 antibody has been validated for WB. It is suitable to detect LEF1 in samples from Human, Mouse, Rat, Dog, Guinea Pig, Cow and Horse.

Quick Overview for LEF1 antibody (N-Term) (ABIN2792635)

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Cow, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LEF1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-LEF1 Antibody

Binding Specificity: N-Term

Sequence: VARQAQTSQE PYHDKAREHP DDGKHPDGGL YNKGPSYSSY SGYIMMPNMN

Cross-Reactivity: Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)

Predicted Reactivity: Cow: 92%, Dog: 92%, Guinea Pig: 85%, Horse: 100%, Human: 100%, Mouse: 85%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against LEF1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human LEF1

Application Details

Application Notes: WB Suggested Anti-LEF1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:312500
Positive Control: Hela cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 399 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for LEF1

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Alternative Name: LEF1

Background: Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1). Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA, MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1, MIM 163905).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: DKFZp586H0919, TCF1ALPHA, LEF-1, TCF10, TCF7L3
Protein Interaction Partner: RNF138, UBC, RNF14, NLK, NARF, STAT5A, PITX2, CTNNB1, CDX1, DPYSL2, nrarp, FHIT, MITF, HINT1, TLE2, TLE1, SETD8, MYB, E2F1, HDAC1, ZBTB3, DACT1, DAZAP2, ALYREF, PIAS4, ALX4, SMAD3, SUMO2, EP300, SMAD1, SMAD2, SMAD4, KPNA1, KPNA2, UBTF, RUNX2, NOTCH1, RUVB
Protein Size: 399

Molecular Weight: 44 kDa

Gene ID: 51176

NCBI Accession: NM_016269, NP_057353

UniProt: Q9UJU2

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding