C-JUN antibody (C-Term)

Catalog No. ABIN2792667

This anti-C-JUN antibody is a Rabbit Polyclonal antibody detecting C-JUN in WB. Suitable for Human, Mouse, Rat, Cow, Dog, Sheep, Zebrafish (Danio rerio) and Guinea Pig.

Quick Overview for C-JUN antibody (C-Term) (ABIN2792667)

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Reactivity: Human, Mouse, Rat, Cow, Dog, Sheep, Zebrafish (Danio rerio), Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C-JUN antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-C-JUN Antibody

Binding Specificity: C-Term

Sequence: EEPQTVPEAR SRDATPPVSP INMEDQERIK VERKRLRNRL AATKCRKRKL

Cross-Reactivity: Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against JUNB. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human JUNB

Application Details

Application Notes: WB Suggested Anti-JUNB Antibody Titration: 2.5 µg/mL
ELISA Titer: 1:62500
Positive Control: Transfected 293T.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 347 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for C-JUN

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Alternative Name: Jun

Background: The c-Jun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus v-Jun oncogene. The c-Jun protein, along with c-Fos, is a component of the AP-1 transcriptional complex. c-Jun can form either Jun/Jun homodimers or Jun/Fos heterodimers via the leucine repeats in both proteins. Jun B and Jun D, have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. JunB is involved in many types of human carcinoma including T-cell lymphomas, CML,primary cutaneous lymphomas. Aberrantly expressed c-Jun and JunB are a hallmark of Hodgkin lymphoma cells, stimulate proliferation and synergize with NF-kappa B. JunB potentiates function of BRCA1 activation domain 1 (AD1) through a coiled-coil-mediated interaction.JunB is an important regulator of erythroid Differentiation.
Alias Symbols: AP-1
Protein Interaction Partner: BATF, FOS, RFWD2, C19orf68, USP24, TCERG1, ZNF595, PKIA, MAP2, APLP2, FOSL1, DDIT3, ATF3, BATF2, BATF3, TCL1A, FOSL2, NPM1, NEU1, SMAD4, HOXA7, BDNF, ATF4, MAPK6, UBC, TDG, SET, SAT1, MAPK9, BRCA1, SUMO2, Cebpb, EP300, SMURF1, YY1, CREBBP, SMARCA4, JDP2,
Protein Size: 347

Molecular Weight: 36 kDa

Gene ID: 3726

NCBI Accession: NM_002229, NP_002220

UniProt: P17275

Pathways: MAPK Signaling, RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling, S100 Proteins