MNAT1 antibody (N-Term)

Catalog No. ABIN2792694

The Rabbit Polyclonal anti-MNAT1 antibody (ABIN2792694) specifically detects MNAT1 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Pig, Dog and Zebrafish (Danio rerio) samples.

Quick Overview for MNAT1 antibody (N-Term) (ABIN2792694)

Target: MNAT1 (Menage A Trois Homolog 1, Cyclin H Assembly Factor (Xenopus Laevis) (MNAT1))

Reactivity: Human, Mouse, Rat, Cow, Pig, Dog, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MNAT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MNAT1 Antibody

Binding Specificity: N-Term

Sequence: DDQGCPRCKT TKYRNPSLKL MVNVCGHTLC ESCVDLLFVR GAGNCPECGT

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chicken, Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against MNAT1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MNAT1

Application Details

Application Notes: WB Suggested Anti-MNAT1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:1562500
Positive Control: 721_B cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 309 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MNAT1

Target: MNAT1 (Menage A Trois Homolog 1, Cyclin H Assembly Factor (Xenopus Laevis) (MNAT1))

Alternative Name: MNAT1 / RNF66

Background: The coenzyme NAD and its derivatives are involved in hundreds of metabolic redox reactions and are utilized in protein ADP-ribosylation, histone deacetylation, and in some Ca(2+) signaling pathways. NMNAT (EC 2.7.7.1) is a central enzyme in NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD.Cyclin-dependent kinases (CDKs), which play an essential role in cell cycle control of eukaryotic cells, are phosphorylated and thus activated by the CDK-activating kinase (CAK). CAK is a multisubunit protein that includes CDK7 (MIM 601955), cyclin H (CCNH, MIM 601953), and MAT1. MAT1 (for 'menage a trois-1') is involved in the assembly of the CAK complex.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-498 AA053721.1 1-498 499-1372 BC000820.1 489-1362 1373-1388 BU620200.1 1-16 c
Alias Symbols: MAT1, RNF66, TFB3, CAP35
Protein Interaction Partner: RPA3, RPA2, RPA1, CDK7, CDK2, MBP, CTD, CCNH, NGFRAP1, UBC, NKX3-1, USP2, SUPT5H, POLR2A, GTF2H4, GTF2H3, GTF2H1, ERCC2, tat, TRIM39, TRIM34, TRIM17, TRIM2, ICK, TRIM31, RNF41, RNF7, TRIM26, MKRN3, TRIM21, MDM4, BRCA1, TRIML1, RNF32, TRIM9, TRIM5, XBP1P1,
Protein Size: 309

Molecular Weight: 36 kDa

Gene ID: 4331

NCBI Accession: NM_002431, NP_002422

UniProt: P51948

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase