MNAT1 antibody (AA 92-278)

Catalog No. ABIN3043583

The Rabbit Polyclonal anti-MNAT1 antibody is suitable to detect MNAT1 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IF, ICC and FACS.

Quick Overview for MNAT1 antibody (AA 92-278) (ABIN3043583)

Target: MNAT1 (Menage A Trois Homolog 1, Cyclin H Assembly Factor (Xenopus Laevis) (MNAT1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MNAT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-MNAT1 Antibody

Binding Specificity: AA 92-278

Purpose: Anti-MNAT1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-MNAT1 Antibody (ABIN3043583). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MNAT1 recombinant protein (Position: L92-A278). Human MNAT1 shares 93% amino acid (aa) sequence identity with mouse MNAT1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MNAT1

Target: MNAT1 (Menage A Trois Homolog 1, Cyclin H Assembly Factor (Xenopus Laevis) (MNAT1))

Alternative Name: MNAT1

Background:

Synonyms: CDK-activating kinase assembly factor MAT1,CDK7/cyclin-H assembly factor,Cyclin-G1-interacting protein,Menage a trois,RING finger protein 66,RING finger protein MAT1,p35,p36,MNAT1,CAP35, MAT1, RNF66,

Tissue Specificity: Highest levels in colon and testis. Moderate levels are present thymus, prostate, ovary, and small intestine. The lowest levels are found in spleen and leukocytes.

Background: CDK-activating kinase assembly factor MAT1 is an enzyme that in humans is encoded by the MNAT1 gene. The protein encoded by this gene, along with cyclin H and CDK7, forms the CDK-activating kinase (CAK) enzymatic complex. This complex activates several cyclin-associated kinases and can also associate with TFIIH to activate transcription by RNA polymerase II. Two transcript variants encoding different isoforms have been found for this gene.

Sequence Similarities: Contains 1 RING-type zinc finger.

Molecular Weight: 36 kDa

Gene ID: 4331

UniProt: P51948

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase