MTOR antibody (pThr2446)

Catalog No. ABIN2794213

This Rabbit Polyclonal antibody specifically detects MTOR in DB. It exhibits reactivity toward Human.

Quick Overview for MTOR antibody (pThr2446) (ABIN2794213)

Target: MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MTOR antibody is un-conjugated

Application: Dot Blot (DB)

Product Details anti-MTOR Antibody

Binding Specificity: pThr2446

Purpose: Rabbit Anti-Human Phospho-FRAP1(T2446) Antibody

Immunogen: This FRAP1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T2446 of human FRAP1.

Isotype: Ig Fraction

Application Details

Application Notes: Dot Blot
Recommended Dilutions
DB: 1:500
Dot blot analysis of anti-FRAP1-pT2446 Phospho-specific Pab on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 μg per ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.450 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C (short-term), -20°C (long-term)

Target Details for MTOR

Target: MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))

Alternative Name: FRAP1

Background:

Target Description: FRAP1 belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. FRAP1 is a part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization.

Gene Symbol: MTOR

Molecular Weight: 288892 Da

Gene ID: 2475

UniProt: P42345

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, BCR Signaling, Warburg Effect