SUMO4 antibody (Val55-Mutant)

Catalog No. ABIN2797851

This anti-SUMO4 antibody is a Rabbit Polyclonal antibody detecting SUMO4 in WB and IHC. Suitable for Human.

Quick Overview for SUMO4 antibody (Val55-Mutant) (ABIN2797851)

Target: SUMO4 (Small Ubiquitin Related Modifier 4 (SUMO4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUMO4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: V55

Product Details anti-SUMO4 Antibody

Binding Specificity: Val55-Mutant

Purpose: Rabbit Anti-Human SUMO4 (V55 Mutant) Antibody

Immunogen: This SUMO4 antibody is generated from rabbits immunized with a KLH conjugated peptide CEPRGLS(V)KQIRFRFG selected from human SUMO4. This antibody is affinity purified using peptides CEPRGLS(V)KQIRFRFG (positive selection) and CEPRGLS(M)KQIRFRFG (negative selection).

Isotype: Ig Fraction

Application Details

Application Notes: Western Blot, Immunohistochemistry
Recommended Dilutions
WB: 1:1000, IHC: 1:50-100Western blot analysis of SUMO4 Antibody (V55 Mutant) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated. BC = breast carcinoma, HC = hepatocarcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.0 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C (short-term), -20°C (long-term)

Target Details for SUMO4

Target: SUMO4 (Small Ubiquitin Related Modifier 4 (SUMO4))

Alternative Name: SUMO4

Background:

Target Description: SUMO4 is a member of the SUMO gene family. This family of small ubiquitin-related modifiers covalently modify target lysines in proteins and control the target proteins' subcellular localization, stability, or activity. Upon oxidative stress, SUMO4 conjugates to various anti-oxidant enzymes, chaperones, and stress defense proteins. This protein may also conjugate to NFKBIA, TFAP2A and FOS, negatively regulating their transcriptional activity, and to NR3C1, positively regulating its transcriptional activity. Covalent attachment to SUMO4 substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I. In contrast to SUMO1, SUMO2 and SUMO3, SUMO4 seems to be insensitive to sentrin-specific proteases due to the presence of Pro-90. This may impair processing to mature form and conjugation to substrates. SUMO4 is located in the cytoplasm and specifically modifies IKBA, leading to negative regulation of NF-kappa-B-dependent transcription of the IL12B gene. The M55V substitution has been associated with type I diabetes.

Gene Symbol: SUMO4

Molecular Weight: 10685 Da

Gene ID: 387082

UniProt: Q6EEV6