EEF2K antibody (pSer359) (AbBy Fluor® 594)

Catalog No. ABIN2812679

This anti-EEF2K antibody is a Rabbit Polyclonal antibody detecting EEF2K in WB, IF (p) and IF (cc). Suitable for Human.

Quick Overview for EEF2K antibody (pSer359) (AbBy Fluor® 594) (ABIN2812679)

Target: EEF2K (Eukaryotic Elongation Factor-2 Kinase (EEF2K))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEF2K antibody is conjugated to AbBy Fluor® 594

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-EEF2K Antibody (AbBy Fluor® 594)

Binding Specificity: pSer359

Predicted Reactivity: Human,Mouse,Rat,Dog,Sheep,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human EEF2k around the phosphorylation site of Ser359

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for EEF2K

Target: EEF2K (Eukaryotic Elongation Factor-2 Kinase (EEF2K))

Alternative Name: EEF2k

Background:

Synonyms: EEF2K phospho S359, p-EEF2K phospho S359, Calcium/calmodulin dependent eukaryotic elongation factor 2, Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase, Calmodulin dependent protein kinase III, cb365, eEF 2 kinase, eEF 2K, EEF2K protein, Elongation factor 2 kinase, Eukaryotic elongation factor 2 kinase, kinase eEF2K, SMEF2K, EF2K_HUMAN.

Background: Eukaryotic elongation factor 2 kinase (EEF2k) previously known as Ca2+/calmodulin dependent protein kinase III, is an abundant cytoplasmic protein highly specific for elongation factor 2 (eEf2). Phosphorylation of eEF2 by eEF2 kinase on specific threonine residues results in the inactivation of eEF-2 and in termination of mRNA translation. The activity of eEF2 kinase is not only dependent upon Ca2+ ions, calmodulin (CaM) and insulin, but is also regulated both negatively and positively via phosphorylation by different protein kinases (AMPK, S6K1, p90 RSK). There is also evidence that eEF-2 phosphorylation is involved in the regulation of cell cycle progression, cellular differentiation, oogensis and malignant tumors.

Gene ID: 29904

Pathways: AMPK Signaling