PRAME antibody (C-Term)

Catalog No. ABIN2840972

The Rabbit Polyclonal anti-PRAME antibody is suitable to detect PRAME in samples from Human. It has been validated for WB, FACS and IHC (p).

Quick Overview for PRAME antibody (C-Term) (ABIN2840972)

Target: PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRAME antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB18763

Product Details anti-PRAME Antibody

Binding Specificity: AA 476-502, C-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This MAPE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-502 amino acids from the C-terminal region of human MAPE.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:2000. IHC-P: 1:50~100. FC: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for PRAME

Target: PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))

Alternative Name: MAPE

Background: MAPE is an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, its gene is also expressed in acute leukemias.

Molecular Weight: 57890

Gene ID: 23532

NCBI Accession: NP_006106, NP_996836, NP_996837, NP_996838, NP_996839

UniProt: P78395

Pathways: Retinoic Acid Receptor Signaling Pathway, Nuclear Hormone Receptor Binding