TGFB1 antibody

Catalog No. ABIN285131

The Goat Polyclonal anti-TGFB1 antibody has been validated for WB, IHC, ELISA, ICC, FACS and Neut. It is suitable to detect TGFB1 in samples from Human.

Quick Overview for TGFB1 antibody (ABIN285131)

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TGFB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS), Neutralization (Neut)

Product Details anti-TGFB1 Antibody

No Cross-Reactivity: Human

Cross-Reactivity (Details): Does not react with human LAP derived TGF beta2 precursor

Characteristics: Affinity purified Goat polyclonal TGF beta 1 antibody
Bioactivity: ND50 is typically 15-30 µg/mL in the presence of 125 ng/mL of recombinant human LAP TGF beta 1 and 0.25 ng/mL TGF beta 1.

Purification: Purified

Endotoxin Level: <10 ng per 1 mg of the antibody as determined by the LAL method.

Immunogen: TGF beta 1 antibody was raised in goat using CHO cell-derived recombinant human LAP (TGF-beta1) as the immunogen.

Isotype: IgG

Application Details

Application Notes: FC: 2.5 µg/10^6 cells, IHC: 5-15 µg/mL, ICC: 5-15 µg/mL, WB: 1 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquidsolution in PBS and NaCl with Trehalose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C until reconstitution. Following reconstitution product may be stored at 4 °C in the short term. For long term storage aliquot and freeze at -20 °C.

Target Details for TGFB1

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Alternative Name: TGF beta 1

Background: Transforming growth factor (sometimes referred to as Tumor growth factor, or TGF) is used to describe two classes of polypeptide growth factors, TGF alpha and TGFBeta.

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints