Cyclin A antibody (Biotin)

Catalog No. ABIN2853583

This anti-Cyclin A antibody is a Mouse Monoclonal antibody detecting Cyclin A in WB and IP. Suitable for Human, Mouse and Rat.

Quick Overview for Cyclin A antibody (Biotin) (ABIN2853583)

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin A antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: E67-1

Product Details anti-Cyclin A Antibody (Biotin)

Specificity: This antibody reacts with Cyclin A (55 kDa).

Purification: Protein-A Agarose Chromatography of hybridoma supernatant.

Immunogen: Bovine recombinant Cyclin A.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 20 µg/mL

Buffer: PBS, pH 7.2 containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for Cyclin A

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Alternative Name: Cyclin A2

Background: Cyclin A is a 60 kDa cyclin that binds both cdk2 and p34cdc2 (cdk1), giving two distinct cyclin A kinase activities, one appearing in G1/S phase, the other in G2/M. The level of cyclin A protein peaks in S-phase, and the activity of the cdk2-cyclin A complexes is essential for DNA replication and progression through S phase. Cyclin A is thus a marker for actively proliferating cells and for cells in S-phase. In normal cells, cdk2-cyclin A complexes associate with PCNA and p21CIPP1/WAF1. In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcrIPPtion factors DRT F1 and E2F, and retinoblastoma protein, p110.Synonyms: CCN1, CCNA, CCNA2, Cyclin A, Cyclin-A2

Gene ID: 890

NCBI Accession: NP_001228

UniProt: P20248

Pathways: PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA