NOTCH2 antibody (C-Term)

Catalog No. ABIN2854343

The Rabbit Polyclonal anti-NOTCH2 antibody has been validated for WB and ChIP-seq. It is suitable to detect NOTCH2 in samples from Human and Mouse. There is 1 publication available.

Quick Overview for NOTCH2 antibody (C-Term) (ABIN2854343)

Target: NOTCH2 (Notch 2 (NOTCH2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOTCH2 antibody is un-conjugated

Application: Western Blotting (WB), ChIP DNA-Sequencing (ChIP-seq)

Product Details anti-NOTCH2 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Rat (Rattus)

Cross-Reactivity (Details): Rat (89 %)

Characteristics: Rabbit polyclonal antibody to NOTCH2 (Notch homolog 2 (Drosophila))
NOTCH2 antibody [C2], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human NOTCH2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference Western blot 1:500-1:3000* ChIP-sequencing Assay-dependent dilution Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceWestern blot1:500-1:3000* ChIP-sequencingAssay-dependent dilution

Comment:

Positive Control: A431 , HeLa , HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS, 40 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-NOTCH2 antibody (ABIN2854343)

Lin, Xie, Lin, Fu, Su, Tong, Chen, Wang, Zhao, Xia, Zhang, Zhang: "Epistatic Association of CD14 and NOTCH2 Genetic Polymorphisms with Biliary Atresia in a Southern Chinese Population." in: Molecular therapy. Nucleic acids, Vol. 13, pp. 590-595, (2018) (PubMed).

Target Details for NOTCH2

Target: NOTCH2 (Notch 2 (NOTCH2))

Alternative Name: NOTCH2

Background: This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development.

Cellular Localization: Cell membrane, Single-pass type I membrane protein , Notch 2 intracellular domain: Nucleus

Molecular Weight: 265 kDa

Gene ID: 4853

Pathways: Notch Signaling, Stem Cell Maintenance