IL12RB1 antibody

Catalog No. ABIN2854451

The Rabbit Polyclonal anti-IL12RB1 antibody has been validated for WB. It is suitable to detect IL12RB1 in samples from Human.

Quick Overview for IL12RB1 antibody (ABIN2854451)

Target: IL12RB1 (Interleukin 12 Receptor beta 1 (IL12RB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL12RB1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-IL12RB1 Antibody

Cross-Reactivity: Human

Characteristics: Rabbit polyclonal antibody to IL-12R beta1(CD212) (interleukin 12 receptor, beta 1)
IL12 Receptor beta1 antibody [N1C2]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human IL12 Receptor beta1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A431

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for IL12RB1

Target: IL12RB1 (Interleukin 12 Receptor beta 1 (IL12RB1))

Alternative Name: interleukin 12 receptor subunit beta 1

Background: The protein encoded by this gene is a type I transmembrane protein that belongs to the hemopoietin receptor superfamily. This protein binds to interleukine 12 (IL12) with a low affinity, and is thought to be a part of IL12 receptor complex. This protein forms a disulfide-linked oligomer, which is required for its IL12 binding activity. The coexpression of this and IL12RB2 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. The lack of expression of this gene was found to result in the immunodeficiency of patients with severe mycobacterial and Salmonella infections. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Cellular Localization: Membrane, Single-pass type I membrane protein

Molecular Weight: 73 kDa

Gene ID: 3594

UniProt: P42701

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation