NOTCH3 antibody (C-Term)

Catalog No. ABIN2854480

Product Details anti-NOTCH3 Antibody

Target: NOTCH3 (Notch 3 (NOTCH3))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOTCH3 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Mouse (100 %), Rat (100 %)

Characteristics: Rabbit polyclonal antibody to Notch 3 (Notch homolog 3 (Drosophila))
Notch 3 antibody [C3], C-term

Purification: Affinity purified by Protein A.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Notch 3. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceWestern blot1:500-1:3000*

Comment:

Positive Control: HeLa

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 5.26 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NOTCH3

Target: NOTCH3 (Notch 3 (NOTCH3))

Alternative Name: Notch 3 (NOTCH3 Products)

Synonyms: CADASIL antibody, CASIL antibody, IMF2 antibody, AW229011 antibody, N3 antibody, hpbk antibody, fa14b08 antibody, notch5 antibody, wu:fa14b08 antibody, notch 3 antibody, NOTCH3 antibody, Notch3 antibody, notch3 antibody

Background: This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).

Cellular Localization: Cell membrane, Single-pass type I membrane protein , Notch 3 intracellular domain: Nucleus

Molecular Weight: 244 kDa

Gene ID: 4854

Pathways: Notch Signaling