RAD51 Homolog B antibody (Center)

Catalog No. ABIN2854538

The Rabbit Polyclonal anti-RAD51 Homolog B antibody has been validated for WB. It is suitable to detect RAD51 Homolog B in samples from Human.

Quick Overview for RAD51 Homolog B antibody (Center) (ABIN2854538)

Target: RAD51 Homolog B (Rad51B)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD51 Homolog B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RAD51 Homolog B Antibody

Binding Specificity: Center

Cross-Reactivity: Mouse (Murine), Cow (Bovine)

Cross-Reactivity (Details): Mouse (86 %), Bovine (91 %)

Characteristics: Rabbit polyclonal antibody to RAD51L1 (RAD51-like 1 (S. cerevisiae))
RAD51B antibody [N1C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human RAD51B. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceWestern blot1:500-1:3000*

Comment:

Positive Control: Raji

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for RAD51 Homolog B

Target: RAD51 Homolog B (Rad51B)

Alternative Name: RAD51L1

Background: The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are evolutionarily conserved proteins essential for DNA repair by homologous recombination. This protein has been shown to form a stable heterodimer with the family member RAD51C, which further interacts with the other family members, such as RAD51, XRCC2, and XRCC3. Overexpression of this gene was found to cause cell cycle G1 delay and cell apoptosis, which suggested a role of this protein in sensing DNA damage. At least three alternatively spliced transcript variants encoding distinct isoforms have been observed.

Cellular Localization: Nucleus

Molecular Weight: 42 kDa

Gene ID: 5890

Pathways: DNA Damage Repair