RAD51 Homolog B antibody (AA 180-260)

Catalog No. ABIN7223904

The Rabbit Polyclonal anti-RAD51 Homolog B antibody (ABIN7223904) specifically detects RAD51 Homolog B in WB, ELISA, IHC and IF. The antibody is reactive with Human, Mouse and Monkey samples.

Quick Overview for RAD51 Homolog B antibody (AA 180-260) (ABIN7223904)

Target: RAD51 Homolog B (Rad51B)

Reactivity: Human, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD51 Homolog B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-RAD51 Homolog B Antibody

Binding Specificity: AA 180-260

Purpose: Rabbit Anti-Rad51B Polyclonal Antibody

Specificity: Rad51B Polyclonal Antibody detects endogenous levels of Rad51B protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Rad51B at AA range: 180-260

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RAD51 Homolog B

Target: RAD51 Homolog B (Rad51B)

Alternative Name: Rad51B

Background: RAD51B, RAD51L1, REC2, DNA repair protein RAD51 homolog 2, R51H2, RAD51 homolog B, Rad51B, RAD51-like protein 1The protein encoded by RAD51B (RAD51 paralog B) is a member of the RAD51 protein family. RAD51 family members are evolutionarily conserved proteins essential for DNA repair by homologous recombination. This protein has been shown to form a stable heterodimer with the family member RAD51C, which further interacts with the other family members, such as RAD51, XRCC2, and XRCC3. Overexpression of RAD51B was found to cause cell cycle G1 delay and cell apoptosis, which suggested a role of this protein in sensing DNA damage. Rearrangements between this locus and high mobility group AT-hook 2 (HMGA2, GeneID 8091) have been observed in uterine leiomyomata.

Molecular Weight: 45kD

Gene ID: 5890

UniProt: O15315

Pathways: DNA Damage Repair