NUF2 antibody

Catalog No. ABIN2854611

The Rabbit Polyclonal anti-NUF2 antibody (ABIN2854611) specifically detects NUF2 in WB, ICC and IF. The antibody is reactive with Human samples.

Quick Overview for NUF2 antibody (ABIN2854611)

Target: NUF2 (NUF2, NDC80 Kinetochore Complex Component (NUF2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-NUF2 Antibody

Characteristics: Rabbit Polyclonal antibody to CDCA1 (kinetochore protein Nuf2)
CDCA1 antibody

Purification: Purified by antigen-affinity chromatography.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Western blot1:1000-1:10000*

Comment:

Positive Control: HeLa nucleus

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.35 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NUF2

Target: NUF2 (NUF2, NDC80 Kinetochore Complex Component (NUF2))

Alternative Name: CDCA1

Background: This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described.

Cellular Localization: Nucleus , Kinetochore

Molecular Weight: 54 kDa

Gene ID: 83540

Pathways: Maintenance of Protein Location