NUF2 antibody

Catalog No. ABIN7117086

The Rabbit Polyclonal anti-NUF2 antibody (ABIN7117086) specifically detects NUF2 in WB, ELISA and IP. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for NUF2 antibody (ABIN7117086)

Target: NUF2 (NUF2, NDC80 Kinetochore Complex Component (NUF2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-NUF2 Antibody

Purpose: NUF2 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: NUF2, NDC80 kinetochore complex component, homolog

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for NUF2

Target: NUF2 (NUF2, NDC80 Kinetochore Complex Component (NUF2))

Alternative Name: NUF2

Background:

Synonyms: Kinetochore protein Nuf2 (hNuf2, hNuf2R, hsNuf2)|Cell division cycle-associated protein 1|NUF2|CDCA1|NUF2R

Background: Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity(PubMed:12438418, PubMed:14654001, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:17535814). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore(PubMed:15548592). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules(PubMed:23085020).

Molecular Weight: 50 kDa

Gene ID: 83540

UniProt: Q9BZD4

Pathways: Maintenance of Protein Location