NQO1 antibody (C-Term)

Catalog No. ABIN2854666

This Rabbit Polyclonal antibody specifically detects NQO1 in WB, IF, ICC and IHC (p). It exhibits reactivity toward Human, Mouse and Ratand has been mentioned in 1 publication.

Quick Overview for NQO1 antibody (C-Term) (ABIN2854666)

Target: NQO1 (NAD(P)H Dehydrogenase, Quinone 1 (NQO1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NQO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NQO1 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Pig (Porcine), Rhesus Monkey, Cow (Bovine), Guinea Pig

Cross-Reactivity (Details): Pig (92 %), Rhesus Monkey (100 %), Bovine (83 %), Guinea pig (100 %)

Characteristics: Rabbit Polyclonal antibody to NQO1 (NAD(P)H dehydrogenase, quinone 1)
NQO1 antibody [C2C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NQO1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: Molt-4 , Raji , mouse heart , rat heart

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-NQO1 antibody (ABIN2854666)

Ishitsuka, Huebner, Rice, Koch, Speransky, Steven, Roop: "Lce1 Family Members Are Nrf2-Target Genes that Are Induced to Compensate for the Loss of Loricrin." in: The Journal of investigative dermatology, Vol. 136, Issue 8, pp. 1656-1663, (2017) (PubMed).

Target Details for NQO1

Target: NQO1 (NAD(P)H Dehydrogenase, Quinone 1 (NQO1))

Alternative Name: NQO1

Background: This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Cellular Localization: Cytoplasm

Molecular Weight: 31 kDa

Gene ID: 1728