14-3-3 sigma/SFN antibody (C-Term)

Catalog No. ABIN2854692

This anti-14-3-3 sigma/SFN antibody is a Rabbit Polyclonal antibody detecting 14-3-3 sigma/SFN in WB, IF, IHC (p) and ICC. Suitable for Human.

Quick Overview for 14-3-3 sigma/SFN antibody (C-Term) (ABIN2854692)

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 sigma/SFN antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-14-3-3 sigma/SFN Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to 14-3-3 sigma (stratifin)
14-3-3 sigma antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human 14-3-3 sigma. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , HeLa , HepG2 , Neuro2A , GL261 , PC-12 , A549

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for 14-3-3 sigma/SFN

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Alternative Name: stratifin

Background: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. p53-regulated inhibitor of G2/M progression.

Cellular Localization: Cytoplasm , Nucleus , Secreted

Molecular Weight: 28 kDa

Gene ID: 2810

UniProt: P31947

Pathways: p53 Signaling, Myometrial Relaxation and Contraction